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          甲型肝炎病毒Vero細胞適應株的選育研究

            [摘要]目的篩選和優化C群腦膜炎多糖的衍化工藝。方法將C群奈瑟腦膜炎球菌多糖經溴化氰活化后通過己二酰肼衍生后制備出適宜的多糖衍生物。對衍化條件進行摸索和優化后通過衍化率及衍生物的可溶性來考察衍化效果,同時通過生化指標來考察該衍生工藝制備的結合疫苗的質量和收率。結果在pH 11.0±0.2、活化30 rain以及溴化氰與多糖比值(w )=0.5時,所獲衍生物制備的結合疫苗各項生化指標在所篩選的條件中最優。結論 經過篩選優化的衍生工藝提高了多糖的衍化率,適于結合疫苗的研制,保證了結合疫苗的免疫原性。
            [關鍵詞】奈瑟腦膜炎球菌;莢膜多糖;結合疫苗;衍化率;多糖衍生物;免疫原性;正交設計法 [中圖分類號】R512.3 [文獻標識碼】A [文章編號】 1006—4028(2005)04—397—04

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