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          CpG ODN聯合乙型肝炎疫苗與乙型肝炎疫苗免疫效果對比研究


          【摘要】 目的 探計CpG ODN乙型肝炎疫苗與市售乙型肝炎疫苗對Balb/e小鼠的免疫作用效果。方法 將乙肝疫苗、乙肝疫苗+100ugCpG ODN 分別肌肉注射到4-6周齡,16-18g Balb/c小鼠合體內,于第1次免疫后28d以同樣劑量加強免疫1次。分別于第1次免疫后28、42、63 d收集小鼠血清,用ELISA方法檢測搞HBs IgG搞體。結果 實驗組產生的抗HBs IgG與對照組HBs IgG相比P<0.05,具有顯著差異。28、42、63 d抗HBs IgG分別是對照疫苗的 114倍、4.73倍、5.41w倍以上。結論 CpG ODN能夠顯著增強小鼠對乙肝疫苗的免疫應答,在較短時間內顯著的提高乙肝抗體的產生水平,免疫效果是乙肚疫苗的5倍以上。
            【關鍵詞】CpG脫氧寡核苷酸;乙肝疫苗;乙肝抗體水平

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